Part:BBa_K3237012:Design

Antisense RNA for NAB1

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The designed were inspired by the bioProtocol from Xie et al., 2014 [3]. The part is made double stranded (oligo-duplex) through a quick thermocycler program. However, it should be noted that the additions of the prefix-suffix ends are not enough to allow for proper cloning according to this protocol. Some modifications to the ends will be needed. This part is based on the NAB1 construct that was codon optimized for C. reinhardtii that is known as BBa_K3237011.

Source

This part was designed by our teammate Luke Saville based on the literature [1,2]. The part was purchased as an oligo pair from IDT.

References

1. Mussgnug, J., Wobbe, L., Elles, I., Claus, C., Hamilton, M., Fink, A., Kahmann, U., Kapazoglou, A., Mullineaux, C., Hippler, M., Nickelsen, J., Nixon, P., and Kruse, O. (2005) NAB1 Is an RNA Binding Protein Involved in theLight-Regulated Differential Expression of theLight-Harvesting Antenna ofChlamydomonas reinhardtii. The plant cell. 17, 3409-3421

2. Beckmann, J., lehr, F., Finazzi, G., Kankamer, B., Posten, C., Wobbe, L., and Kruse, O. (2009) Improvement of light to biomass conversion by de-regulation of light-harvesting protein translation in Chlamydomonas reinhardtii. Journal of Biotechnology. 142, 70-77

3. Xie, K., Minkenberg, B., and Yang, Y. (2014) Targeted Gene Mutation in Rice using a CRISPR-Cas9 System. Bio-Protocol. 4(17): e1225. DOI: 10.21769/BioProtoc.1225.